Page 14 - CUA2018 Abstracts - Oncology-Prostate

Poster session 1: Prostate Cancer I

UP–1.13 established stable LC3 double tagged (mCherrry–GFP) lines and observed
Impact of autophagy in development of prostate cancer resistance the dynamic of autophagic flux using confocal microscopy and flow
3,4
Maxime Cahuzac 1,3,4 , Benjamin Péant , Anne–Marie Mes–Masson 1,3,4 , cytometry. Finally, we determined the effect of autophagy inhibition, using
Fred Saad 2,3,4 siRNA against Atg7, on the proliferation and survival of PCa cell lines
2
1 Médecine, Université de Montréal, Montreal, QC, Canada; Chirurgie, exposed to docetaxel.
3
Université de Montréal, Montreal, QC, Canada; Cancer, CRCHUM, Results: We observed varying basal levels of autophagy in the four PCa
4
Montreal, QC, Canada; Institut du Cancer de Montréal, Montreal, QC, cell lines, with hormono–sensitive cells (LNCaP, 22Rv1) demonstrating a
Canada lower intrinsic autophagy as compared to hormono–resistant lines (PC3,
Introduction: Patients with metastatic prostate cancer (PCa) have improved DU145). Analyses of double–tagged LC3 cells showed that docetaxel
outcome when treated with chemotherapy, but over time, develop che- increases the autophagic flux in all cell lines, although this is greatest in
moresistance and succumb to the disease. Recently, it has been shown the hormono–sensitive cells. An inhibition of autophagy reduces the pro-
that chemotherapeutic agents can induce the activation of autophagy liferation and survival of all PCa cell lines after treatment with docetaxel.
promoting resistance, although this has yet to be studied in PCa. Here, Conclusions: These results suggest that hormone sensitivity of cell lines
we characterize autophagy in several PCa cell lines exposed to chemo- appears to correlate with the basal level of autophagy. We confirm that
therapeutics used in the clinic. autophagy levels are increased following exposure to chemotherapeu-
Methods: Levels of autophagy were measured by PCR and Western blot tic agents. A strategy that combines agents inhibiting autophagy with
analysis in four PCa cell lines (LNCaP, 22Rv1, PC3 and DU145) both chemotherapeutics may further improve outcome of patients with PCa.
before and after exposure to either docetaxel or cabazitaxel. Next, we

S72 CUAJ • June 2018 • Volume 12(6Suppl2)