Page 14 - CUA Presentation

PARP Biology

DNA
Damage

PARP plays a key role in the repair of ssDNA

breaks via base excision repair (BER) pathway
NAD+

• Binds directly to sites of DNA damage

Nicotinamide + • Once activated, uses NAD as a substrate
pADPr
to add large, branched chains of

pADPr polymers (ie, PARylation)

• Recruits other DNA repair enzymes

to site of damage

ADP, adenosine diphosphate; Lig, ligase; NAD, nicotinamide adenine dinucleotide; pADPr, poly(adenosine diphosphate ribose); PARP, poly(ADP-ribose) polymerase; PNK, polynucleotide kinase; Pol, polymerase

Ohmoto A, Yachida S. Onco Targets Ther 2017;10:5195-208.