Page 6 - Flipbook

PARP Biology

PARP plays a key role in the repair of ssDNA

DNA damage breaks via base excision repair (BER) pathway
PARP

• Binds directly to sites of DNA damage

NAD+

• Once activated, uses NAD as a substrate

Nicotinamide + to add large, branched chains of pADPr
pADPr polymers (ie, PARylation)

XRCC1 Lig3 • Recruits other DNA repair enzymes to
PARP
PNK site of damage
Polß

ADP, adenosine diphosphate; Lig, ligase; NAD, nicotinamide adenine dinucleotide; pADPr, poly(adenosine diphosphate ribose); PARP, poly(ADP-
ribose) polymerase; PNK, polynucleotide kinase; Pol, polymerase
Ohmoto A, Yachida S. Onco Targets Ther 2017;10:5195-208.